Studying the Effect of Culture Conditions on Cordyceps pruinosa (Petch, 1924) Biomass
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Abstract
Cordyceps pruinosa (Petch, 1924) is an entomopathogenic fungus rich in valuable bioactive compounds; however, data on its biological characteristics and biomass cultivation in Vietnam remain limited. This study aimed to investigate the environmental conditions in a static liquid culture system by evaluating the mycelial biomass yield of a native strain isolated from Bidoup-Nui Ba National Park. Using a one-factor-at-a-time (OFAT) approach, the optimal culture conditions were identified: temperature at 25 ± 1 °C, medium pH of 6.0, sucrose as the ideal carbon source, peptone as the most suitable organic nitrogen source, vitamin B₁ at 0.1 g/L, and a harvest time of 25 days. Under these conditions, the maximum dry biomass yield reached 6.77 ± 0.11 g/L (coefficient of variation = 1.62%). These findings provide a foundation for standardizing the culture medium for larger-scale production and support further research on this fungal species.
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